the human erythrocyte is a rich raw material for the purification of cu-zn superoxide dismutase (sod). we applied a simple and rapid procedure for the purification of sod from human erythrocytes by ion exchange chromatography. the purified sod had a specific activity of 2285.6 u/mg protein and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sd...

background: non-steroidal anti-inflammatory drugs (nsaid) have been associated with antioxidant property and have been shown to improve the circulating antioxidant status on daily dosing in different inflammatory conditions. the present study was conducted to investigate the antioxidant role of meloxicam in aluminum induced oxidative stress in rat brain. methods: in the in vivo experiments, spr...

introduction : recurrent aphthous stomatitis (ras) is a common disease with unknown etiology and no treatment. the purpose of this study is to assess the relationship between serum antioxidants level and clinical manifestation. change in antioxidant level can lead inflammatory reaction through oxidative stress. materials & methods : a total 50 patients were selected for this cross sectional stu...

Two proteins (P1 and P2, with weights of 57,500 and 27,500 respectively) were isolated from Euglena gracilis. Both proteins show cyanide-insensitive superoxide dismutase activity in the "classical" superoxide dismutase assay, using xanthine-xanthine oxidase as O2.- generator. If O2.- is generated chemically (autoxidation of reduced anthraquinone), photochemically (illuminated riboflavine) or pu...

Superoxide dismutases (SODs) represent the major antioxidant defense system against superoxide anions (O2 ). Three isoforms of superoxide dismutase have been identified in mammals: copper-zinc SOD (Cu, ZnSOD), manganese SOD (Mn-SOD), and extracellular SOD (ecSOD). Cu, Znand Mn-SOD are localized intracellularly, whereas the last discovered SOD isoform, ecSOD,1 is secreted and bound to heparan su...

Distribution profiles of superoxide dismutase isoenzymes in various tissues of rabbits with the Vx-2 carcinoma in the maxillary sinus were compared with those of control rabbits. Copper- and zinc-containing superoxide dismutase (Cu,Zn-SOD) activity in the liver of rabbits decreased significantly 3 weeks after transplantation. Manganese-containing superoxide dismutase (Mn-SOD) activity did not d...

Superoxide dismutase (SOD)-deficient Escherichia coli OX326A cells are protected against chemically-induced oxidative stress by expression of the chaperonin GroESL. This protection is equivalent to expression of superoxide dismutase even though GroESL has no inherent SOD activity. Co-overexpression of GroESL and SOD in the same cells results in higher protein yields of SOD and greater metallati...

The phototrophic bacterium Rhodobacter capsulatus contains a single, oxygen-responsive superoxide dismutase (SOD(Rc)) homologous to iron-containing superoxide dismutase enzymes. Recombinant SOD(Rc), however, displayed higher activity after refolding with Mn(2+), especially when the pH of the assay mixture was raised. SOD(Rc) isolated from Rhodobacter cells also preferentially contains manganese...

Enzymatic superoxide-dismutase activity is believed to be important in defense against the toxic effects of superoxide. Although superoxide dismutases are among the best studied proteins, numerous questions remain concerning the specific biological roles of the various superoxide-dismutase types. In part, this is because the proposed damaging effects of superoxide are manifold, ranging from ina...